To determine
To Find: Function is one to one.
Answer
given function is not one to one.
Explanation
fx=x4-16
Horizontal Line Test says that a function f is one to one if and only if any horizontal line intersects the graph of given function exactly at one point. In other words, the Horizontal Line Test can be stated as:

A function f is NOT one to one if and only if there exists a horizontal line which intersects the graph of given function at least at two point.
Conclusion:
Since any horizontal line (except at the point of minima) will intersect the given graph more than once, by using the Horizontal Line Test, we can conclude that the given function is not one to one.
Note: If you have to say that the given function is not one to one by Horizontal Line test, then existence of just one horizontal line, which intersects the given graph more than once, is sufficient.